Growth Promoting Substance in Yeast Extract for Methylotrophic Growth ofCandida boidinii
نویسندگان
چکیده
منابع مشابه
Characterization of chromatographic yeast extract fractions promoting CHO cell growth
Background Many studies underline the great benefits of yeast extracts (YE), used as supplements in animal free culture media, on cell growth and recombinant protein production [1,2]. Nevertheless, their unknown composition and batch-to-batch variability of commercial YE remain constraints for industrial processes [3,4]. Consequently, the identification of bioactive YE molecules is challenging ...
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The vertical elongation of normal roots of Lupinus seedlings proceeds at constant rate over periods of 4 to 5 hours. The decapitation of a root stops its elongation for a variable length of time, followed by a period of renewed elongation at a rate lower than that of the normal root. The tipping of the decapitated root with a tip of a coleoptile of Zea induces a decrease in the rate of elongati...
متن کاملPeroxisomal catalase in the methylotrophic yeast Candida boidinii: transport efficiency and metabolic significance.
In this study we cloned CTA1, the gene encoding peroxisomal catalase, from the methylotrophic yeast Candida boidinii and studied targeting of the gene product, Cta1p, into peroxisomes by using green fluorescent protein (GFP) fusion proteins. A strain from which CTA1 was deleted (cta1Delta strain) showed marked growth inhibition when it was grown on the peroxisome-inducing carbon sources methano...
متن کاملPhysiological role of S-formylglutathione hydrolase in C(1) metabolism of the methylotrophic yeast Candida boidinii.
The methylotrophic yeast Candida boidinii exhibits S-formylglutathione hydrolase activity (FGH, EC 3.1.2.12), which is involved in the glutathione-dependent formaldehyde oxidation pathway during growth on methanol as the sole carbon source. The structural gene, FGH1, was cloned from C. boidinii, and its predicted amino acid sequence showed more than 60 % similarity to those of FGHs from Paracoc...
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The structural gene (FDH1) coding for NAD(+)-dependent formate dehydrogenase (FDH) was cloned from a genomic library of Candida boidinii, and the FDH1 gene was disrupted in the C. boidinii genome (fdh1 delta) by one-step gene disruption. In a batch culture experiment, although the fdh1 delta strain was still able to grow on methanol, its growth was greatly inhibited and a toxic level of formate...
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 2006
ISSN: 0916-8451,1347-6947
DOI: 10.1271/bbb.60144